Plasmid

Part:BBa_I713021:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pBL-Blunt


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2198
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2515
    Illegal XhoI site found at 2157
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2198
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2198
    Illegal NgoMIV site found at 2404
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1291
    Illegal SapI site found at 208
    Illegal SapI site found at 2344


Design Notes

By digesting with PstI and SphI and blunting.


Source

BBa_I713022

References